This pipeline enables users to quickly and accurately identify a mapping interval from Arabidopsis thaliana mapping populations, and then identify homozygous mutations within that mapping interval. Specifically, this pipeline is specialized for a bulk segregant population between Col-0 and Ler ecotypes, Col-0 being the ecotype in which the mutant was identified.
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Cuperus JT, Montgomery TA, Fahlgren N, Burke RT, Townsend T, Sullivan CM, Carrington JC. (2010) Identification of MIR390a precursor processing-defective mutants in Arabidopsis by direct genome sequencing. Proc Natl Acad Sci USA 107, 466-71